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文献和实验(to ensure nucleoprotein complex dissociation). 5. Add 1 ml of 1-Bromo-3-chloropropane (BCP)(for per 10 ml TriReagent) and shake vigorously for 15 sec. 6. Incubate at RT for 2-15 minutes (Average 10 minutes). 7. Centrifuge homogenate at 3200 rpm for 30
on microtiter shaker at speed of 4 until a clear lysate is developed, takes approximately 15 minutes. 6.Add 300 μl 3M NaOAc, pH 4.5 to each well using the Hydra. Shake in the HiGro at 37degC and a shaker setting of 400 rpm for 30 minutes. 7.Freeze the blocks
) to each well, using the Hydra. Incubate on microtiter shaker at speed of 4 until a clear lysate is developed, takes approximately 15 minutes. 6.Add 300 μl 3M NaOAc, pH 4.5 to each well using the Hydra. Shake in the HiGro at 37degC and a shaker setting
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