- 询价
- 05-457
- 2025年07月15日
- Western Blotting;Immunocytochemistry
- Mouse
- Bovine;Human;Green Plants
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Mouse
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Bovine;Human;Green Plants
- 是否单克隆：
1
- 规格：
200 μg
Product Images
WB: 20 micrograms of HeLa cell acid extract was probed with Anti-Histone H1, clone AE-4 (5μg/ml).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, B, Pl | ELISA, IC, WB | Mouse | Purified | Monoclonal Antibody |
- H1 histone family, member 4
- Histone H1b
- histone 1, H1e
- histone cluster 1, H1e
- ELISA
- Immunocytochemistry
- Western Blotting
5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.
ELISA:
Recommended.
- Human
- Bovine
- Green Plants
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
- HIST1H1E
- H1F4
- H1.4
- dJ221C16.5
- MGC116819
Western Blot:
1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
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Nuclei from myeloid leukemia biopsy cells 详细描述见链接：http://www.millipore.com/catalogue/item/05-457
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文献和实验Histone Assay Solution (10 rxns) Amount [Final] Histone Wash Buffer 320 µL - Histone H1, 2 µg/µL 18 µl 0.1 µg/µL
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
) or presence (+) of okadaic acid (500 nM; Calbiochem), an inhibitor of cellular phosphatases. Acid‐soluble nuclear proteins and isolated histone H1 were subjected to electrophoresis in a TAU gel and analyzed by Coomassie blue staining. Lane M contains
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