- 询价
- 05-443
- 2025年07月14日
- Immunoprecipitation;Immunohistochemistry
- Mouse
- Human;Mouse;Rat;Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Immunohistochemistry
- 宿主：
Mouse
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Human;Mouse;Rat;Rabbit
- 是否单克隆：
1
- 规格：
100 μg
Product Images
IHC: Paraffin-embedded human breast carcinoma stained with anti-VEGF at 10μg/ml
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rb | IH(P), Blocking of Inteferring Antibodies, not WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-VEGF, clone JH
Background Information:
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Alternate Names:
- Vascular permeability factor
- vascular endothelial growth factor
- vascular endothelial growth factor A
- vascular endothelial growth factor isoform VEGF165
Clone:
clone JH
Specificity:
Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).
Key Applications:
- Immunohistochemistry (Paraffin)
- Blocking of Inteferring Antibodies
Applications Not Recommended:
Western Blotting
Application Notes:
Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Species Reactivity:
- Human
- Mouse
- Rat
- Rabbit
Species Reactivity Note:
Human, rabbit, mouse, and rat. Other species not tested.
Isotype:
IgG1κ
Immunogen:
Recombinant human VEGF121. Clone JH121
Molecular Weight:
34-54 kDa isoforms
Control:
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Presentation:
Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.
Storage Conditions:
Stable for 1 year at from date of receipt.
Gene Symbol:
- VEGFA
- VEGF-A
- VEGF
- VPF
- MGC70609
Quality Assurance:
Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.
Antibody Category:
Signaling
Antibody Sub-Category:
Growth Factors & Receptors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein G chromatography
Product Family Information
VEGF Antibodies & ProteinsMillipore’s Anti- VEGF antibody demonstrates specificity against VEGF. See below for data, references and related products for VEGF. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Recombinant human VEGF 详细描述见链接：http://www.millipore.com/catalogue/item/05-443
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文献和实验相关实验
地命中目标一样。另一方面，即使是同一个抗原决定簇，在机体内也可以由好几种克隆来产生抗体，形成好几种单克隆抗体混杂物，称为多克隆抗体。在抗原抗体反应中，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免）。 2、应用范围的选择。有的一抗只能用于 WB ( Western blotting）或免疫组化、免疫荧光、免疫沉淀等；甚至表明石蜡切片或冰冻切片。 3、种属反应性的选择。这一点很重
应用。用GST融合表达系统表达外源基因时，需要对融合表达的产物进行检测和纯化，这里面就包括了GST标签抗体的应用，如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源，比如小鼠源的单克隆抗体还是兔源的多克隆抗体，一般来说，单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6]（货号#E022040）是来源于小鼠的单克隆抗体，克隆号为GST.B6，免疫原为GST多肽 。
能目标明确地与单一的特异抗原决定簇结合，就像导弹精确地命中目标一样。另一方面，即使是同一个抗原决定簇，在机体内也可以由好几种克隆来产生抗体，形成好几种单克隆抗体混杂物，称为多克隆抗体。在抗原抗体反应中，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免）。 2.应用范围的选择。有的一抗只能用于 WB ( Western blotting）或免疫组化、免疫荧光、免疫沉淀等；甚至表明
技术资料暂无技术资料 索取技术资料
Anti-VEGF单克隆抗体 人鼠兔大鼠适用 免疫沉淀 免疫组化 抗血管内皮生长因子
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