Anti-Clusterin α chain (human)

Anti-Clusterin α chain (human), clone 41D

Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.

• Apolipoprotein J
• Complement cytolysis inhibitor
• Complement-associated protein SP-40,40
• Ku70-binding protein 1
• Testosterone-repressed prostate message 2
• aging-associated protein 4
• clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
• complement lysis inhibitor
• complement-associated protein SP-40
• sulfated glycoprotein 2

41D

This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.

• Immunocytochemistry
• Immunohistochemistry
• Immunohistochemistry (Paraffin)
• Western Blotting

Immunohistochemistry:
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.

Human

Human.

IgG1κ

Native clusterin purified from human serum. Clone 41D.

40 kDa

HeLa cell lysate.

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• CLU
• SP-40
• MGC24903
• NA1/NA2
• SGP-2
• AAG4
• KUB1
• APOJ
• CLI
• TRPM-2
• TRPM2
• Apo-J
• SGP2

Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates

• Apoptosis & Cancer
• Neuroscience

• Apoptosis - Additional
• Neurodegenerative Diseases

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

1 mg/mL

Monoclonal Antibody

Purified

Mouse