- 询价
- 04-002
- 2026年07月11日
- Immunoprecipitation;Western Blotting
- Mouse
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Western Blotting
- 宿主：
Mouse
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Human;Mouse
- 是否单克隆：
1
- 规格：
100 μg
Product Images
Immunoblot Analysis Lysate from KG-1 cells was resolved by electrophoresis, transferred to PVDF, and probed with anti-SOCS1 for 2 hours at room...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Mouse | Purified | Monoclonal Antibody |
- Immunoprecipitation
- Western Blotting
Immunoprecipitation (IP): 10 μg of this antibody immunoprecipitated SOCS1 from 200 μL of cell extract in RIPA buffer.
- Human
- Mouse
- SOCS1
- SSI-1
- Cish1
- TIP-3
- TIP3
- CISH1
- SSI1
- CIS1
- SOCS-1
- JAB
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
mouse SOCS1 recombinant GST fusion protein encompassing amino acids 2-78. 详细描述见链接：http://www.millipore.com/catalogue/item/04-002
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文献和实验policeman.4. Put the extract into an eppendorf and boil for 5 min., cool on ice.5. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60 mm dish. This is about 100 μg protein for PC12 cells.6. After electrophoresis, electrotransfer
Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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