STEMdiff™ Kidney Organoid Kit is a complete, serum-free cell culture medium system that supports highly efficient and reproducible generation of human pluripotent stem cell (hPSC)-derived kidney organoids in a simple, two-stage differentiation protocol. These kidney organoids are composed of podocytes, proximal tubules, distal tubules, as well as the associated endothelium and mesenchyme.
Kidney organoids generated with STEMdiff™ Kidney Organoid Kit are tested for compatibility with phenotypic high-throughput assays such as nephrotoxic compound screening. They also provide a relevant and convenient model for studies related to developmental biology and disease modeling.
Advantages: ⦁ RELEVANT. Enables generation of human kidney organoids that model the developing nephron and associated endothelium and mesenchyme.
⦁ SIMPLE. Minimizes culture manipulations with a two-stage culture system and easy-to-follow protocol.
⦁ RELIABLE. Provides low experimental variability through an optimized formulation and rigorous quality controls.
⦁ HIGH THROUGHPUT. Reproducibly generates organoids in 96- and 384-well formats.
Components:
STEMdiff™ Kidney Basal Medium, 100 mL
STEMdiff™ Kidney Supplement SG (100X), 200 µL
STEMdiff™ Kidney Supplement DM (50X), 1.6 mL
Contains: Serum-free medium kit for the culture of kidney orgaonids from hPSCs