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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Protein-lysine 6-oxidase (Lox)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Protein-lysine 6-oxidase (Lox)品名简称:
Recombinant Rat Lox protein货号:
CSB-EP013038RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
LoxUniprot No.:
P16636别名:
Lysyl oxidase种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
33 kDa表达区域:
163-411aa氨基酸序列:
DDPYNPYKYSDDNPYYNYYDTYERPRSGSRHRPGYGTGYFQYGLPDLVPDPYYIQASTYVQKMSMYNLRCAAEENCLASSAYRADVRDYDHRVLLRFPQRVKNQGTSDFLPSRPRYSWEWHSCHQHYHSMDEFSHYDLLDASTQRRVAEGHKASFCLEDTSCDYGYHRRFACTAHTQGLSPGCYDTYAADIDCQWIDITDVQPGNYILKVSVNPSYLVPESDYSNNVVRCEIRYTGHHAYASGCTISPY蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin.功能2:
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin文献:
Metalloproteinase activity secreted by fibrogenic cells in the processing of prolysyl oxidase. Potential role of procollagen C-proteinase.Panchenko M.V., Stetler-Stevenson W.G., Trubetskoy O.V., Gacheru S.N., Kagan H.M.J. Biol. Chem. 271:7113-7119(1996)研究领域:
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文献和实验OLR1(LOX-1)配体结合机制、胞内信号调控及蛋白试剂应用解析
。此外,OLR1在多种免疫及特定功能细胞中也广泛存在,包括单核吞噬细胞、中性粒细胞、树突状细胞、滋养层细胞以及胶质细胞。这种广泛的分布模式提示OLR1不仅在血管生物学中发挥作用,还深度参与了免疫调节及神经系统的生理过程。 OLR1的结构和其受体 OLR1(又称LOX-1)是一种属于C型凝集素超家族的II型跨膜糖蛋白,分子量约50-52 kDa,由273个氨基酸组成。其结构主要包含三个部分:N端的短胞质尾区、跨膜结构域以及C端的胞外凝集素样结构域,后者是识别配体的关键区域。作为受体,OLR1的主要配体是氧
,从 1995 年获得美国癌症学会研究奖开始,他斩获无数大奖,2015 年达到顶峰,获得美国国立卫生研究院杰出研究奖,并且当选为美国医学科学院院士。正所谓成也科研,败也科研,从 2016 年开始,陆续有人指出 Amato J. Giaccia 教授的多篇论文涉嫌造假,其中包括一篇主刊和四篇子刊,下面一起来看看这几篇文章。第一篇文章于 2006 年发表在 nature 杂志上,目前已经被引用 1202 次。《Lysyl oxidase is essential for hypoxia-induced
有 ie-1、ie-2、lef类基因、p35、pe38等。2、杆状病毒载体表达系统的特点 AcNPV病毒用作外源基因的表达载体,通常是通过体内同源重组的方法,用外源基因替代多角体蛋白基因而构建重组病毒。由于多角体基因启动子在感染后18~24h开始转录和翻译,一直持续到70 h。外源基因置换掉多角体基因后,并不影响后代病毒的感染与复制,意味着重组病毒不需要辅助病毒的功能。 杆状病毒表达系统自从第一次用来表达干扰素以后在许多重组蛋白的表达中得到广泛应用,例如用于表达白介素(IL)一2,3、BMP及多种病毒蛋白等。相对
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