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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Pyruvate kinase isozymes R/L (Pklr)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Pyruvate kinase isozymes R/L (Pklr)品名简称:
Recombinant Rat Pklr protein货号:
CSB-EP018071RA规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PklrUniprot No.:
P12928别名:
L-PKPyruvate kinase isozymes L/R种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length来源:
E.coli分子量:
66.2 kDa表达区域:
1-574aa氨基酸序列:
MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Plays a key role in glycolysis.功能2:
Plays a key role in glycolysis.文献:
The L- and R-type isozymes of rat pyruvate kinase are produced from a single gene by use of different promoters.Noguchi T., Yamada K., Inoue H., Matsuda T., Tanaka T.J. Biol. Chem. 262:14366-14371(1987)研究领域:
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文献和实验to a 1.5 mL tube (40µL x # of reactions) Spin down beads, aspirate supernatant, resuspend in 1 mL of RIPA. Resuspend beads in 1 vol. of RIPA (40µL x # of reactions) Vortex beads before adding 30 µL to each reaction (a lot
cells per sample. Cells in 1 ml of the 2% FCS, DMEM, transfer to an Eppendorf tube and stimulate with agonists of choice, for the required time and temperature. 3. Spin for 5 - 10 min @ 1000x g at room temperature. Remove the supernate and add 500 µl
Compartmentalized Protein Kinase C Activation in Ovarian Carcinoma Cells
Protein kinase C (PKC), a family of phospholipid-dependent serine/threonine kinases, plays a cardinal role in malignancy (1 -5 ). PKC isozymes can be categorized into three groups: Group A or conventional (c) PKC: α, βI, βII, and γ; Group B
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