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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Paralemmin-1 (PALM)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Paralemmin-1 (PALM)品名简称:
Recombinant Human PALM protein货号:
CSB-EP017413HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PALMUniprot No.:
O75781别名:
Paralemmin种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
45.7 kDa表达区域:
1-384aa氨基酸序列:
MEVLAAETTSQQERLQAIAEKRKRQAEIENKRRQLEDERRQLQHLKSKALRERWLLEGTPSSASEGDEDLRRQMQDDEQKTRLLEDSVSRLEKEIEVLERGDSAPATAKENAAAPSPVRAPAPSPAKEERKTEVVMNSQQTPVGTPKDKRVSNTPLRTVDGSPMMKAAMYSVEITVEKDKVTGETRVLSSTTLLPRQPLPLGIKVYEDETKVVHAVDGTAENGIHPLSSSEVDELIHKADEVTLSEAGSTAGAAETRGAVEGAARTTPSRREITGVQAQPGEATSGPPGIQPGQEPPVTMIFMGYQNVEDEAETKKVLGLQDTITAELVVIEDAAEPKEPAPPNGSAAEPPTEAASREENQAGPEATTSDPQDLDMKKHRCKCC蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner.功能2:
Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner.文献:
"A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).研究领域:
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文献和实验子而不是生物大分子。因此,STORM 需要利用荧光发色团标记抗体对靶蛋白进行识别,可以检测到内源性蛋白,避免 PALM 中由于外源性表达偶联荧光蛋白的靶蛋白对其定位产生的可能的影响。 受激发射损耗显微术 STED 是一种基于点扩散函数调制的超分辨技术,最早由 2014 年诺贝尔奖得主之一 Hell 提出。STED 采用两束共轴激光,分别为激发光和损耗光。在激发光的照射下,基态的荧光分子吸收光子跃迁到激发态并发射出荧光;再使用环形损耗光将第 1 束光斑周围的荧光物质通过受激损耗过程灭活,只保留位于中间
从而提高分辨率。相同的基本概念也很好地支持了Eric Betzig 和 Samuel Hess的研究,尽管他们依靠活化和永久脱色的光敏探针而不是依靠光控开关。 2006年Zhuang的小组在《自然方法》上发表了他们的想法,称他们的为随机光重建显微镜(STORM),并使用它以20纳米的分辨率观测DNA 和DNA蛋白质复合体。与此同时,Lippincott-Schwartz,Betzig, 和 Harald Hess在《科学》上发表了他们的PALM方法,使用它里给细胞黏着斑和特定细胞器的蛋白
光学重建显微镜(stochastic optical reconstruction microscopy, STORM),以及(荧光)光敏定位显微技术(PALM, photoactivated localization microscopy),利用这些方法可以达到生物样品成像10-100nm的分辨率,从而令研究人员观察到亚细胞结构的具体细节。 但是由于大部分蛋白分子只有几纳米,如果要直接解析细胞中这些分子的相互作用,还需要更高的分辨率。在这篇文章中,研究人员就介绍了一种实验
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