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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
PA200 antibodies were generated against a partial fragment encompassing residues 840 –1140 Arabidopsis thaliana protein sequence UniProt:F4JC97-1, TAIR: At3g13330
- 形态:
Lyophilized
- 保存条件:
Store lyophilized/reconstituted at -20°C (short te
- 克隆性:
Polyclonal
- 标记物:
Book et al. (2010). Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes. J Biol Chem. 2010 Aug 13;285(33):25554-69. doi: 10.1074/jbc.M110.136622.
- 适应物种:
Arabidopsis thaliana
- 抗原来源:
F4JC97-1
- 级别:
分子生物学级
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
1 : 500 (WB)
- 靶点:
PA200 (Proteasome activator PA200) is an associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during DNA damage response. It also recognizes and bi
- 抗体英文名:
Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.
- 抗体名:
PA200 | Proteasome activator PA200
- 规格:
50 µl
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文献和实验The 26S proteasome is composed of the core 20S proteasome in association with the 19S regulatory complex, or PA700. PA700 has multiple activities, including ATPase activity, polyubiquitin-chain binding activity, deubiquitination activity
Atomic Force Microscopy of Proteasome Assemblies
. However, only a single structure of the core particle decorated with PA26 activator has been determined. NMR spectroscopy was successfully applied to probe a much �simpler, archaebacterial type of the core particle. In turn, electron microscopy was very effective
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
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