- 询价
- Agrisera
- 瑞典
- AS09 577
- 2026年03月19日
- 1 : 1000-1 : 3000 (WB)
- Goat
- Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
- 形态：
Lyophilized
- 保存条件：
Store lyophilized/reconstituted at -20°C; once rec
- 克隆性：
Polyclonal
- 标记物：
McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.
- 适应物种：
Arabidopsis thaliana, Avena strigosa, Nicotiana tabacum, Solanum lycopersicum
- 抗原来源：
Q39258
- 级别：
分子生物学级
- 供应商：
Agrisera
- 宿主：
Goat
- 应用范围：
1 : 1000-1 : 3000 (WB)
- 靶点：
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic secto
- 抗体英文名：
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to in
- 抗体名：
V-ATPase | Epsilon subunit of tonoplast H+ATPase (goat antibody)
- 规格：
300 µl
Application example
6 µg of total SDS-extracted protein from Avena strigosa roots (R) and leaves (L) , were separated on NuPage LDS-PAGE 4-12% gradient acrylamide gel (Invitrogen) and blotted 1h to nitrocellulose. Filters were blocked 1h with 5% low-fat milk powder in TBS and probed with anti-V-ATPase antibodies (AS09 577 , 1:2000, 1h) and secondary anti-goat (1:5000, 1 h) antibody in TBS containing 5% low fat milk powder. Antibody incubations were followed by washings in TBS-T (containing 0.05% Tween-20, 0.1% Triton X-100) . All steps were performed at RT with agitation. Blots were scanned with a Typhoon scanner.
Courtesy Dr. Sam Mugford (JIC), UK
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to cytochrome oxidase (Complex IV), the relative levels of all OxPhos enzyme complexes in normal and diseased tissues can be evaluated. The mouse monoclonal 7H10 anti�OxPhos Complex V subunit (bovine) (anti�F1 F0 -ATPase subunit , A21350) and mouse monoclonal
） 五类(Class)：（ H链C区的不同氨基酸组成和排列顺序） IgG --- γ(gamma) / 330aa IgA --- α(alpha) / 330aa IgM --- μ(mu) / 440aa IgD --- δ(delta) / 330aa IgE ---ε(epsilon) / 440aa 亚类(Subclasses)：（ 同一类IgH链C区，氨基酸组成的较小差异/二硫键位置或数目不同） IgG：IgG1, IgG
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