Donkey anti-Sheep IgG (H&L), H
RP conjugated, min. cross-reactivity to human, mouse,rabbit IgG- 询价
- Agrisera
- 瑞典
- AS10 992
- 2025年11月18日
- 1 : 200-1 : 5000 (WB) 1 : 500-1 : 5000 (IHC)
- Donkey
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
Lyophilized
- 保存条件：
Store lyophilized material at 2-8°C.For long time
- 克隆性：
Polyclonal
- 级别：
分子生物学级
- 供应商：
Agrisera
- 宿主：
Donkey
- 应用范围：
1 : 200-1 : 5000 (WB) 1 : 500-1 : 5000 (IHC)
- 靶点：
Donkey anti-sheep IgG (H&L) is a HRP conjugated secondary antibody which binds to sheep IgG (H&L). It is especially adsorbed against human, mouse, rabbit IgG. Antibodies are affinity purified using solid phase sheep IgG.
- 抗体名：
Donkey anti-Sheep IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse,rabbit IgG
- 规格：
1 mg
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文献和实验blots. In direct ELISAs, this antibody shows less than 2% cross-reactivity with human IgG and rabbit IgG. Optimal dilutions should be determined by each laboratory for each application. 谢谢!!!
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
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