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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Properdin (Cfp)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Properdin (Cfp)品名简称:
Recombinant Mouse Cfp protein货号:
CSB-EP005291MO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CfpUniprot No.:
P11680别名:
Complement factor P种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
51.9 kDa表达区域:
23-464aa氨基酸序列:
SDPVLCFTQYEESSGRCKGLLGRDIRVEDCCLNAAYAFQEHDGGLCQACRSPQWSAWSLWGPCSVTCSEGSQLRHRRCVGRGGQCSENVAPGTLEWQLQACEDQPCCPEMGGWSEWGPWGPCSVTCSKGTQIRQRVCDNPAPKCGGHCPGEAQQSQACDTQKTCPTHGAWASWGPWSPCSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGPAYEHKACSGLPPCPVAGGWGPWSPLSPCSVTCGLGQTLEQRTCDHPAPRHGGPFCAGDATRNQMCNKAVPCPVNGEWEAWGKWSDCSRLRMSINCEGTPGQQSRSRSCGGRKFNGKPCAGKLQDIRHCYNIHNCIMKGSWSQWSTWSLCTPPCSPNATRVRQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGTPRPLCEALQGQKLVVEEKRSCLHVPVCKDPEEKKP蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.功能2:
A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.文献:
"Properdin, the terminal complement components, thrombospondin and the circumsporozoite protein of malaria parasites contain similar sequence motifs."Goundis A., Reid K.B.M.Nature 335:82-85(1988)研究领域:
Immunology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验validation),也是抗体业仅有的 1,000 多个通过「敲除验证」的高品质商业抗体!第二种情况,相对复杂,需要引用别人已发表的敲除验证的数据。请注意,并不是引用的文献越多越好,而是要有至少一篇证明抗体特异性的文献。以下以发表在 Cell Report 上 CFP1 靶点研究文章4 为例。这篇文章研究的是不孕症里的一种卵母细胞不能完成发育的情况,涉及到基因信息的传递和表观遗传学的机制。其中组蛋白修饰 Histone modifications 是卵母细胞在发育中的关键步骤,该研究应用了卵母细胞的 CFP
(Fluorescence Resonance Energy Transfer, FRET)技术在活细胞生理条件下无损伤的研究蛋白质-蛋白质间相互作用》1.FRET基本原理荧光共振能量转移是指两个荧光发色基团在足够靠近时,当供体分子吸收一定频率的光子后被激发到更高的电子能态,在该电子回到基态前,通过偶极子相互作用,实现了能量向邻近的受体分子转移(即发生能量共振转移)。下面以GFP的两个突变体CFP(cyan fluorescent protein)、 YFP(yellow
光。两个发色基团之间的能量转换效率与它们之间的空间距离的6次方成反比,对空间位置的改变非常灵敏。例如要研究两种蛋白质a和b间的相互作用,可以根据FRET原理构建融合蛋白,这种融合蛋白由三部分组成:CFP(cyan fluorescent protein)、蛋白质b、YFP(yellow fluorescent protein)。用CFP吸收波长433nm作为激发波长,实验灵巧设计,使当蛋白质a与b没有发生相互作用时,CFP与YFP相距很远不能发生荧光共振能量转移,因而检测到的是CFP的发射波长为476
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