- 询价
- Agrisera
- 瑞典
- AS20 4380
- 2025年11月01日
- 1: 500 - 1: 2000 (Dot), (ELISA), (IP), 1 : 1000 - 1: 3000 (WB)
- Mouse
- DYKDDDDK (Sigma FLAG®)
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store at -20°C; avoid repeated freeze-thaw cycles.
- 克隆性：
Monoclonal
- 适应物种：
DYKDDDDK (Sigma FLAG®)
- 抗原来源：
UniProt
- 级别：
分子生物学级
- 供应商：
Agrisera
- 宿主：
Mouse
- 应用范围：
1: 500 - 1: 2000 (Dot), (ELISA), (IP), 1 : 1000 - 1: 3000 (WB)
- 靶点：
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
- 抗体英文名：
Antibody detects N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins and 1-5 ng of FLAG tagged proteins in Western blot using chemiluminescent detection.
- 抗体名：
DYKDDDDK (binds to Sigma FLAG®) (mouse antibody, monoclonal)
- 规格：
100 µg
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文献和实验Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
≥1 μl (as required) Platinum® Taq Antibody: Taq DNA polymerase 1 μl 2.5 units (or as required
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