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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified normal IgA isolated from pooled rat serum,
- 形态:
Purified by salt precipitation, followed by a ion-exchange chromatography with DEAE Sepharose, in PBS pH 7.2.
- 保存条件:
Store at +4°C, for long time storage, store at -20°C.
- 克隆性:
Polyclonal
- 适应物种:
Rat IgA Fc
- 供应商:
Agrisera
- 宿主:
Goat
- 应用范围:
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
- 靶点:
Goat anti-Rat IgA (Fc specific), HRP-conjugated
- 抗体英文名:
Goat anti-Rat IgA (Fc specific), HRP-conjugated
- 抗体名:
Goat anti-Rat IgA (Fc specific), HRP-conjugated
- 规格:
10 mg
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文献和实验小鼠抗IgA抗体(anti-IgA-Ab)酶联免疫分析(ELISA)
-IgA-Ab) ,再与HRP 标记的抗原结合,形成抗原 - 抗体 - 抗原复合物 ,经过彻底洗涤后 加 底物TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 抗IgA 抗体 (anti-IgA-Ab) 呈正相关。用酶标仪在 450 nm波长下测定吸光度( OD 值), 通过标准曲线 计算样品 中 小鼠 抗IgA 抗体 (anti-IgA-Ab) 浓度。 试剂盒组成 : 试剂
Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries
system unless presented as an epitope conjugated to a suitable immunogenic carrier protein, such as bovine thyroglobulin. The principal interest in anti-hapten Abs is as detection molecules for use in diagnostic assays. These typically use dipstick (qualitative
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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