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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
V5 tag sequence GKPIPNPLLGLDST
- 形态:
Immunogen affinity purified in 100 mM Sodium Phosphate (pH 7.4), 100 mM NaCl, 50 mM Sucrose, 1.5% BSA and 0.01% Thimerosal.
- 保存条件:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
- 克隆性:
Polyclonal
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Immunolocalization (IL)
- 靶点:
Anti-V5, DyLight® 550 conjugated
- 抗体英文名:
Anti-V5, DyLight® 550 conjugated
- 抗体名:
Anti-V5, DyLight® 550 conjugated
- 规格:
100 µg (1mg/ml)
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文献和实验Anti-claudin-4, whose corresponding antigen receptors are known to be overexpressed in both primary and metastatic human pancreatic cancer, is utilized for targeted delivery and imaging of pancreatic cancer. In this protocol
:NuPAGE® 凝胶可在250V下跑24分钟 标准运行步骤 为了获得有效的分离,传统Laemmli凝胶需要跑90分钟。相比之下,NuPAGE® 凝胶的标准步骤则为200V,35分钟。此外,如果使用加速步骤,仅需24分钟就能获得有效分离(250V)。 图2. 加快运行时间,而不牺牲性能。NuPAGE® 样品上样:(1) Mark12™ 未染色标准品,10 μL;(2) 大鼠肝裂解液,10 μg;(3) 溶菌酶,6 μg;(4) Bio
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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