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- 文献和实验
- 技术资料
- 保存条件:
待更新
- 保质期:
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- 英文名:
Anti-Mouse IgG Magnetic Bead
- 库存:
Anti-Mouse IgG Magnetic Bead
- 供应商:
艾美捷
- CAS号:
Anti-Mouse IgG Magnetic Bead
- 规格:
1mL
产品名称:http://www.amyjet.com/news/abnova-updates.shtml-Anti-Mouse IgG Magnetic Bead
产品货号:U0011-E
产品规格:1mL
背景资料:待更新
保存建议:http://www.amyjet.com/news/abnova-updates.shtml-Anti-Mouse IgG Magnetic Bead待更新
产品描述:待更新
点击:http://www.amyjet.com/news/abnova-updates.shtml-Anti-Mouse IgG Magnetic Bead
关键词:生化试剂,抗小鼠IgG磁珠,待更新
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文献和实验Mouse B cell isolation with magnetic beads
实验概要 Isolate B cells from total mouse cells Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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