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- 保存条件:
待更新
- 保质期:
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- 英文名:
DEPC-treated Water
- 库存:
DEPC-treated Water
- 供应商:
艾美捷
- CAS号:
DEPC-treated Water
- 规格:
500mL
产品名称:http://www.amyjet.com/news/abnova-updates.shtml-DEPC-treated Water
产品货号:R0012-E
产品规格:500mL
背景资料:待更新
保存建议:http://www.amyjet.com/news/abnova-updates.shtml-DEPC-treated Water待更新
产品描述:待更新
点击:http://www.amyjet.com/news/abnova-updates.shtml-DEPC-treated Water
关键词:生化试剂,经DEPC处理的水,待更新
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
, use DEPC-treated water to generate the solution. Although H2 O prepared with reverse osmosis systems like Milli-Q is free of RNases (Huang 1995) , it can be contaminated by microbial growth if the machine and piping are not well maintained
RNase and DEPC Treatment: Fact or Laboratory Myth
(0.1% and 1%). It would be impossible to predict the different interactions of DEPC with all molecular biology reagents. The most cautious approach for making RNase-free solutions would be to mix molecular biology grade powdered reagents up in DEPC-treated water. Alternatively
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