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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine Kappa-casein (CSN3)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bovine Kappa-casein (CSN3)品名简称:
Recombinant Bovine CSN3 protein货号:
CSB-EP006064BO规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CSN3Uniprot No.:
P02668别名:
/种属:
Bos taurus (Bovine)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
26.4 kDa表达区域:
22-190aa氨基酸序列:
QEQNQEQPIRCEKDERFFSDKIAKYIPIQYVLSRYPSYGLNYYQQKPVALINNQFLPYPYYAKPAAVRSPAQILQWQVLSNTVPAKSCQAQPTTMARHPHPHLSFMAIPPKKNQDKTEIPTINTIASGEPTSTPTTEAVESTVATLEDSPEVIESPPEINTVQVTSTAV蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May regulate immune response to the intracellular capsid in acting as a T-cell tolerogen, by having an immunoregulatory effect which prevents destruction of infected cells by cytotoxic T-cells. This immune regulation may predispose to chronicity during perinatal infections and prevent severe liver injury during adult infections.功能2:
/文献:
"Nucleotide sequence of a cloned hepatitis B virus genome, subtype ayr: comparison with genomes of the other three subtypes." Okamoto H., Imai M., Shimozaki M., Hoshi Y., Iizuka H., Gotanda T., Tsuda F., Miyakawa Y., Mayumi M. J. Gen. Virol. 67:2305-2314(1986)研究领域:
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文献和实验一种含有微量糖的磷蛋白。是乳蛋白的主要成分。乳中酪蛋白含量(%):人为 0.9,马为 1.3,牛为 2.5(约相当于牛乳总蛋白的 80%)。在等电点 pH4.6附近不溶于水,通常利用这种性质,使脱脂牛乳在弱酸下,生成的沉淀于中性附近溶于水,再使之在酸性条件下沉淀,如此反复操作来制造纯品。此标准样品(酸酪蛋白)在电泳上仍是不一致的,可分为α -酪蛋白、κ -酪蛋白和β -酪蛋白。各种酪蛋白具有容易聚合的性质,单体分子量为 2— 3万。每一种都含磷。此外还知有γ -酪蛋白。酪蛋白含有所有的氨
越少,做过外泌体的朋友都知道,每减少一次纯化步骤,就能获取更多的外泌体,每增加一步,就会大幅度降低外泌体的得率。 比如超离得率 15%-20%、沉淀法得率 35%-40%,等等。 因此,就 迫切需要一款无血清、低蛋白的化学成分确定的培养基 来满足 MSC 干细胞外泌体的上游细胞培养需要。 其他的培养体系到底有什么问题? Fetal Bovine Serum(FBS) 一直是细胞培养的霸主,客户一般选用的培养体系是:普通 MSC 细胞培养基+FBS,营养是比较足的,但是对于外泌体研究却面临
owen79 各位大侠好,小弟愚问一下,我想测定NF-KB活化后细胞核内的量,这需要提取核蛋白,但是蛋白量太少,提取出来能否测出NF-KB啊!thank you ! ronaldo_zj 1. 通过提取细胞核、细胞浆内的蛋白来行western blot检测NF-kappa B 的表达情况是可以的,因为很多时候是通过NF-kappa B转位来实现活化的,所以提取的时候我不知道楼主是自己配制试剂提取还是用试剂盒提取的,我建议
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