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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Cytochrome c oxidase subunit 1 (MT-CO1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Cytochrome c oxidase subunit 1 (MT-CO1), partial品名简称:
Recombinant Human MT-CO1 protein, partial货号:
CSB-EP015072HU规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
MT-CO1Uniprot No.:
P00395别名:
Cytochrome c oxidase polypeptide I (COI) (COXI) (MTCO1)种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
36.3 kDa表达区域:
474-513aa氨基酸序列:
EAFASKRKVLMVEEPSMNLEWLYGCPPPYHTFEEPVYMKS蛋白标签:
N-terminal 6xHis-GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.功能2:
/文献:
"Lineage-specific selection in human mtDNA: lack of polymorphisms in a segment of MTND5 gene in haplogroup J." Moilanen J.S., Finnila S., Majamaa K. Mol. Biol. Evol. 20:2132-2142(2003)研究领域:
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文献和实验亦称细胞色素氧化酶。在细胞呼吸中处于细胞色素系统的末端。此酶是把呼吸底物的电子经过细胞色素系统直接传递给分子态氧(即具有自动氧化作用)。 EC1. 9. 3. 1。通常用还原型细胞色素 c的氧催化氧化的作用来进行活性测定。凯林( D. Keilin, 1930)在心肌提取物中首先发现此酶的活性。以前认为是靛酚氧化酶所催化,后来明确了靛酚反应是与细胞色素 c和细胞色素 c氧化酶二者都有关系。从那以后,凯林在心肌和酵母中发现与一氧化碳结合的一种细胞色素(细胞色素 a3 ),并认为
Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
Analysis of Human Mitochondrial DNA Mutations
-cytochrome c oxidoreductase), three to Complex IV (cytochrome c oxidase or COX), and two to Complex V (ATP synthase). These subunits are synthesized within the mitochondrion, where they are assembled together with a larger number of subunits encoded
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