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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃/-80℃
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Elongin-A3 member D (ELOA3DP), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥14796.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥4374.0 |
| 规格: | 20ug | 产品价格: | ¥2328.0 |
Research Areas
OthersUniprot ID
A6NLF2Gene Names
ELOA3DPAlternative Name(s)
/Lead Time
13-23 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
270-429aaProtein Length
PartialTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
LWDPSEAWMQANYDLLSAFEAMTSQANPEALSAPTLQEEAAFPGRRVNAKMPVYSGSRPACQLQVPTLRQQCLRVPRNNPDALGDVEGVPYSALEPVLEGWTPDQPYRTEKDNAALARETDELWRIHCLQDFKEEKPQEHESWRELYLRLRDAREQRLRVMW
25.9 kDaPurity
Greater than 90% as determined by SDS-PAGE.Endotoxin
Not test.Biological_Activity
/Form
Liquid or Lyophilized powderBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.Relevance
/Function
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文献和实验即SRP在内质网膜上的受体蛋白,它能够与结合有信号序列的SRP牢牢地结合,使正在合成蛋白质的核糖体停靠到内质网上来。停靠蛋白含有两个亚基,一个亚基暴露于细胞质的亲水部分,由640个氨基酸组成;另一个亚基是嵌入膜内的疏水部分,由300个氨基酸所组成。SRP受体蛋白除了同SRP结合将核糖体引导到内质网, 同时,它的α亚基与SRP一起催化GTP水解释放能量,帮助信号肽转位。
2和与抗原加工有关的基因称之为低分子量多肽或称大的多功能蛋白酶LMP2(low molecular weight polypeptides or large multifunctional protease-2)、LMP7也位于Ⅱ类基因区。Ⅲ类基因区包括补体C2、C4、B因子,此外,21羟化酶A与B、HSP70(heat shock protein70,热休克蛋白70)和肿瘤坏死因子α、β基因也在这里。21A是假基因,21B具有编码21羟化酶功能。21羟化酶是肾上腺皮质合成皮质醇和醛固醇必不可少的酶
or target protein)。如果在Snf1 和Snf2 之间存在相互作用,那么分别位于这两个融合蛋白上的DB 和AD 就能重新形成有活性的转录激活因子,从而激活相应基因的转录与表达。这个被激活的、能显示“ 诱饵” 和“ 猎物” 相互作用的基因称之为报道基因(reporter gene)。通过对报道基因表达产物的检测,反过来可判别作为“ 诱饵” 和“ 猎物” 的两个蛋白质之间是否存在相互作用。在此Fields 等人采用编码β - 半乳糖苷酶的LacZ 作为报道基因,并且在该基因的上游调控区引入受Gal
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