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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Glutathione S-transferase P (GSTP1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Glutathione S-transferase P (GSTP1)品名简称:
Recombinant Human GSTP1 protein货号:
CSB-EP009989HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GSTP1Uniprot No.:
P09211别名:
GST class-piGSTP1-1种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
28.2 kDa表达区域:
2-210aa氨基酸序列:
PPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.功能2:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.文献:
Structure and expression of a human class pi glutathione S-transferase messenger RNA.Kano T., Sakai M., Muramatsu M.Cancer Res. 47:5626-5630(1987)研究领域:
Metabolism请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验产生大量的可溶性的融合蛋白。谷胱甘肽S-转移酶(GST)、麦芽糖结合蛋白(MBP)以及硫氧还蛋白(Trx)均已经被证实能非常成功地生产正确折叠、有生物活性的蛋白质,能明显提高在E.coli细胞质中产生的融合蛋白的可溶性,并能抑制包涵体的形成[159,194]。其中每一种都备有方便的纯化方法,可将融合蛋白与细胞污染物分开。已经建立了多种对融合蛋白进行位点特异性裂解的方法,方法的选择通常由特定蛋白的组成、序列及物理性质决定[199]。可采用诸如溴化氰(Met↓)、羟胺(Asn↓Gly)、等试剂或低pH
Lazzeri, P. A. and Jepson, I. (2001 ) The expression of amaize glutathione S-transferase gene in transgenic wheat confers herbicide tolerance, both in planta and invitro. M o l. B reed . 7, 301-315. 83. DeVeylder, L ., Van
的噬菌体进行测序分析,找到与靶蛋白结合的目标肽段,进行寻找含该肽段的大蛋白分子,寻找可能和靶蛋白有相互作用的蛋白质分子,为阐明靶蛋白功能提供有效线索。 GST pull-down实验 基于谷胱甘肽巯基转移酶(glutathione S-transferase,GST)融合蛋白的GST pull-down实验是一个行之有效的在蛋白分子水平直接验证蛋白蛋白相互作用的体外实验技术。GST pull-down实验基本原理是将靶蛋白-GST融合蛋白亲和固化在谷胱甘肽亲和树脂上,作为与目的蛋白
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