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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Glutathione S-transferase P (GSTP1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Glutathione S-transferase P (GSTP1)品名简称:
Recombinant Human GSTP1 protein货号:
CSB-EP009989HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GSTP1Uniprot No.:
P09211别名:
GST class-piGSTP1-1种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
28.2 kDa表达区域:
2-210aa氨基酸序列:
PPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.功能2:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.文献:
Structure and expression of a human class pi glutathione S-transferase messenger RNA.Kano T., Sakai M., Muramatsu M.Cancer Res. 47:5626-5630(1987)研究领域:
Metabolism请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验科研人必看:多肽、蛋白质、重组蛋白分不清? 应用场景有哪些?定制如何选?
动物细胞表达:最接近天然状态的表达平台,提供完整的人源化翻译后修饰 (如糖基化、磷酸化),适合重组抗体、细胞因子等需要复杂修饰的蛋白。 昆虫细胞表达:基因承载容量大,支持大分子量蛋白和激酶的表达。 『多种融合标签』 标签的选择直接影响纯化效率和下游应用,MCE 支持多种融合标签,可根据实验需求灵活搭配。 常规纯化标签:His 标签 (镍柱纯化,分子量小)、GST 标签 (Glutathione 亲和纯化,兼具促溶作用)、Fc 标签 (Protein A/G 亲和纯化,适合免疫相关实验)、Flag 标签
产生大量的可溶性的融合蛋白。谷胱甘肽S-转移酶(GST)、麦芽糖结合蛋白(MBP)以及硫氧还蛋白(Trx)均已经被证实能非常成功地生产正确折叠、有生物活性的蛋白质,能明显提高在E.coli细胞质中产生的融合蛋白的可溶性,并能抑制包涵体的形成[159,194]。其中每一种都备有方便的纯化方法,可将融合蛋白与细胞污染物分开。已经建立了多种对融合蛋白进行位点特异性裂解的方法,方法的选择通常由特定蛋白的组成、序列及物理性质决定[199]。可采用诸如溴化氰(Met↓)、羟胺(Asn↓Gly)、等试剂或低pH
Lazzeri, P. A. and Jepson, I. (2001 ) The expression of amaize glutathione S-transferase gene in transgenic wheat confers herbicide tolerance, both in planta and invitro. M o l. B reed . 7, 301-315. 83. DeVeylder, L ., Van
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