AgriseraTMB HRP Substrate(10 m
l)/AgriseraTMB HRP Substrate(10 ml)/AgriseraTMB HRP Substrate(10 ml)- 询价
- Agrisera
- 瑞典
- AS15-TMB-HRP-10-E
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stable for 3 years at +2-8° C or at room temperature up to six months, and can also be frozen. Storge at room temperature for 1 month results in ca. 2 % activity loss. Protect the substrate from exposure to sunlight.
- 保质期：
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- 英文名：
AgriseraTMB HRP Substrate(10 ml)
- 库存：
AgriseraTMB HRP Substrate(10 ml)
- 供应商：
艾美捷
- 规格：
10ml
保存建议：Agrisera-重组蛋白Stable for 3 years at +2-8° C or at room temperature up to six months, and can also be frozen. Storge at room temperature for 1 month results in ca. 2 % activity loss. Protect the substrate from exposure to sunlight.
背景资料：3,3',5,5'-Tetramethylbenzidine, horseradish peroxidase (AS15 TMB-HRP) is a sensitive reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. It is designed to detect and quantify specific peptide/protein/hormone in a complex mixture in enzyme linked immunosorbent assay (ELISA). It utilizes the enzymatic reaction triggered by horse raddish peroxidase (HRP) in which a chromogenic, chemiluminiescent or chemifluorescent substrates are oxidized resulting in color change, chemiluminiscence or chemifluorescence respectively. A one-electron oxidation product is formed in the presence of HRP and hydrogen peroxide. This is a cation free radical, it is blue in color with adsorption maximum at 653 nm. The a reaction with HRP, hydrogen peroxide or acidification of the radical with acid yields the diimine terminal oxidation product that adsorbs light at 450 nm. The extinction coefficient of the radical ( E653 nm = 3.9 x 104 mol-1 cm-1 ) and diimine ( E450 nm = 5.9 x 104 mol-1 cm-1 ) provides a very sensitive system for this assay.After incubations with antibodies or HRP labeled reagents, TMB solution is added. As an alternative TMB can also be spiked with a different volume of buffered HRP to create dilution series of this substrate, for example 50 %, 70%. This will change sensitivity of this product and can make it more suitable for certain assays, depending what kinetics are required. If you require to adjust sensitivity of your assay, please check Agrisera TMB/Diluent pack. The oxidation of TMB by HRP produces a blue reaction product, measured at 650 nm. Color formation can be recorded as a function of time or the reaction can be stopped using an equal volume of 0.3 M sulfuric acid after a fixed interval. Increased sensitivity is achieved by converting the blue radical to the yellow diimine by addition of acid. The resulting yellow chromogen is measured immediately at 450 nm.
点击：Agrisera-重组蛋白查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询Agrisera中国区域授权总代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看每日生物评论网站。
Agrisera公司提供1000种用于植物和藻类细胞生物学研究的一抗，2000多种二抗。Agrisera公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿、烟草、稻、菜豆、小立碗藓、杨树、豌豆、番茄、马铃薯、菠菜、小麦、玉米、葡萄等多个物种，此外还有水生生物类（藻类、鱼）抗体，细菌、昆虫、真菌抗体。针对植物蛋白质的保守氨基酸序列，Agirsera还独家开发了数十种通用抗体（global antibodies），这类抗体几乎可以与所有的高等植物及藻类产生免疫反应，可为研究人员提供极为方便的选择。
艾美捷科技有限公司，通过提供完整的产品和服务体系、便利的客户关系和供应链管理，为生命科学研究工作者提供整体打包方案。我们的企业的核心价值观：“激情、敬业、学习、创新、合作、卓越”。激发我们内在的创造力，提供有竞争力的生物医学产品和服务，持续为客户创造最大价值是我们的企业使命。我们的企业愿景是成为受人尊重、世界知名的生物医学产品及服务供应商。艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系，目前已成为众多国际知名品牌的中国总代理或一级代理，主要包括：AmyJet、AAT Bioquest、Abnova、Agrisera、Biovision、Biosensis、Cayman Chemical、Caisson Labs、Cytoskeleton、CytoNiChe、Epigentek、Equitech-Bio、Hycult Biotech、Jackson、LifeSensors、ProSpec、Norgen Biotek、Mabtech、Matreya、iRegene、StressMarq、ImmunoReagents、SouthernBiotech、Origene、US Biological、Solis BioDyne等，可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。艾美捷服务升级，全方位实验服务项目上线啦~
关键词：AgriseraTMB HRP Substrate(10 ml)/AgriseraTMB HRP Substrate(10 ml)/AgriseraTMB HRP Substrate(10 ml)，AgriseraTMB HRP底物（10毫升）/AgriseraTMB HRP底物（10毫升）/AgriseraTMB HRP底物（10毫升），AgriseraTMB HRP Substrate
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文献和实验as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow the color to develop for 100 minutes and stop reaction by adding 10 µl of 1 N NaOH. Read absorbance
Whole mount antibody staining of zebrafish embryos for markers of segmentation
1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps. Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking at 4oC. 2. Wash with 5 ml 0.1% BSA in PBS for 10 minutes. Wash 3X with 5 ml PBS
for HRP conjugates. Normally a 1:1000 dilution is sufficient. 7. Wash four times as before and then add 100 µl substrate to each well. For AP conjugates: Substrate solution is p - nitrophenyl phosphate (Sigma) at 1 mg/ml in substrate buffer. Allow
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