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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bovine Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2)品名简称:
Recombinant Bovine ITIH2 protein货号:
CSB-EP011895BO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ITIH2Uniprot No.:
P56651别名:
/种属:
Bos taurus (Bovine)蛋白长度:
Full Length来源:
E.coli分子量:
21.3 kDa表达区域:
1-50aa氨基酸序列:
LHYQEVKWRKLGSYEHRLHLKPGRLAKHELEVFNGYFVHFPAPENMIPIG蛋白标签:
N-terminal 6xHis-KSI-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.功能2:
/文献:
"A serum-derived hyaluronan-associated protein (SHAP) is the heavy chain of the inter alpha-trypsin inhibitor." Huang L., Yoneda M., Kimata K. J. Biol. Chem. 268:26725-26730(1993)研究领域:
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文献和实验SWISSPROT数据库检索,分值最高的Adseverin (Scinderin) (Gelsolin-like protein)为37分,也不能正确鉴定。PeptIdent所检索到的Q02566 MYH6_MOUSE Myosin heavy chain, cardiac muscle alpha isoform (MyHC-alpha). - Mus musculus (Mouse).虽然匹配肽段数达到了7个,但由于序列覆盖率仅为6.8%,仍然得不到正确鉴定。综合上述结果,不能鉴定PRO2的蛋白质种属。
10.21 华中科技大学 Nature 子刊揭示精母细胞中逆转录转座子沉默的分子机制
大学和汉阳大学的研究人员发现 G 蛋白α- 13(Gα13)的表达在糖尿病小鼠和人类的肝脏中下降了。在小鼠中仅剔除肝脏中的 Gna13 基因可导致全身性葡萄糖耐受不良。本研究表明小鼠中的全身胰岛素抵抗是由 Gα13 下调导致的肝脏中 ITIH1 过度分泌引起的,这可通过 ITIH1 中和加以逆转。这些发现可能有助于开发治疗代谢疾病的新策略。原文检索:Overproduction of inter-α-trypsin inhibitor heavy chain 1 after loss of Gα13
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
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