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- 询价记录
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant SARS-CoV-2 (2019-nCoV) NSP15
- CAS号:
YUCHUN202011
- 保质期:
2年
- 供应商:
上海瓦兰生物
- 保存条件:
-20°
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥2200.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥3600.0 |
| 规格: | 1mg | 产品价格: | ¥10800.0 |
产品名称(Product name) Recombinant SARS-CoV-2 (2019-nCoV) NSP15
描述(description) Recombinant SARS-CoV-2 NSP15 is produced by E.coli expression system and the target gene encoding Gln6452-Gln6798 is expressed with N-His Tag
表达系统(Expression system) E.coli
种属(Species) Sev ere acute resp iratory syndro me coronav irus 2 (SARS-CoV-2) Accession
分子别名(Alternative names) Uridylate-specific endoribonuclease
预测分子量(Predicted Molecular Mass) 41.24kDa
实际分子量(Actual Molecular Mass) 42kDa
纯度(Purity) >90% as determined by SDS-PAGE
内毒素(Endotoxin level) Please contact with the lab for this information
制剂(Formulation) Supplied as solution form in PBS, pH7.5/ Supplied as lyophilized from PBS, pH7.5
运输方式(Shipping) In general, proteins are shipped out with blue ice unless customers require otherwise.
稳定性&储存(Stability &Storage) Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 °C for one week . Store at -20 to -80 °C for twelve months from the date of receipt.
复溶(Reconstitution) Please refer to the instraction in the hard copy of COA.
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
-ligate ), the antibiotic resistance gene will allow bacteria carrying empty vector plasmids to survive the selection.Transformation The reaction used to create recombinant DNA molecules (ligation reaction, etc.) is used to transform bacteria
Recombinant expression and SDS
Gels 15% 4% 30% Acrylamide 5 ml 0.65 ml 4X Tris-HCl, pH 8.8 2.5 ml 4X Tris-HCl pH 6.8 1.25ml ddH2O 2.5 ml 3.0 ml 10% Ammonium Persulfate 33 ml 50 ml TEMED 7 ml 7 ml SDS electrophoresis buffer, 5× 15.1 g
Heavy Labeling of Recombinant Proteins
Because of the cost of isotopic chemicals and heterologous proteins to produce, an economical 15 N/13 C isotopic labeling method is critically needed. Four protocols have been tested for the expression of Ovine interferon-τ in Pichia pastoris
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