BioLegend 793906 Biotinylated

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BioLegend 793906 Biotinylated Recombinant SARS-CoV-2 S Protein RBD (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：793906
产品名称：BioLegend 793906 Biotinylated Recombinant SARS-CoV-2 S Protein RBD (carrier-free)
产品规格：100 µg

产品说明：
Regulatory Status  RUO
Other Names  S1 RBD, Spike Protein RBD

描述
SARS-CoV-2是一种导致2019guan状病毒病（新冠肺炎）的呼吸道病毒。冠状病毒刺突（S）糖蛋白是病毒粒子外壳上的一种I类病毒融合蛋白，通过识别宿主细胞受体并介导病毒和细胞膜的融合，在病毒感染中发挥关键作用。S糖蛋白是由约1300个氨基酸组成的前体蛋白，然后被切割成氨基（N）末端S1亚基（约700个氨基酸）和羧基（C）末端S2亚基（600个氨基酸）。三个S1/S2异二聚体组装形成从病毒包膜突出的三聚体刺突。S1亚基包含一个受体结合结构域（RBD），该结构域可以特异性结合靶细胞上的受体血管紧张素转化酶2（ACE2）。通过受体结合、蛋白水解处理和/或细胞区室中的酸性pH触发，I类病毒融合蛋白在感染期间经历从亚稳定的融合前状态到稳定的融合后状态的转变，其中受体结合亚基被切割，融合亚基进行大规模构象重排，暴露疏水性融合肽，诱导形成六螺旋束，使病毒和细胞膜接近融合。由三个S1-S2异二聚体组成的三聚体SARS guan状病毒（SARS-CoV-2）S糖蛋白结合细胞受体血管紧张素转化酶2（ACE2），并通过融合前到融合后的构象转变介导病毒和细胞膜的融合。
SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.

Product Details
Source
SARS-CoV-2 S Protein RBD, amino acid Arg319-Phe541 (Accession # QHD43416.1), with a C-terminal 8-his tag and an Avi-tag was expressed in 293E cells. SARS-CoV-2 S Protein RBD was site-specifically biotinylated by enzyme BirA.

Molecular Mass
The 260 amino acid recombinant protein has a predicted molecular mass of approximately 28.9 kD. The DTT-reduced and non-reduced proteins migrate at approximately 35 kD and 30 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Arg.

Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method

Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
When recombinant ACE2-Fc chimera (Cat. No. 793202) is immobilized at 1 µg/mL, biotinylated recombinant SARS-CoV-2 S Protein RBD binds in a dose-dependent manner. The EC50 range for this effect is 8 - 40 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application   Bioassay

Antigen Details
Structure   Biotinylated
Distribution   SARS-CoV-2

Function
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection
Interaction   Lung cells
Ligand/Receptor   ACE2

Bioactivity
Measured by its ability to bind recombinant human ACE2
Biology Area   COVID-19

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