Mouse IgG1 isotype control [MO

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

immunofluorescence staining

allotype or sometimes isotype. Typically antibodies against human antigens and made in mice so are mouse immunoglobulins usually mouse IgG1. The typical second step reagent would be goat anti-mouse IgG conjugated to FITC. However, care should be taken

流式试剂选择及常见问题解答

对照应该是APC标记的Mouse IgG1,κ，货号是555751。注意同型对照的成份跟它的名称是相同的.如果是抗体的组合形式是纯化的一抗＋荧光标记的二抗，那么应该选择一抗的同型对照。比如CDw125的纯化抗体，货号是555901，成分是Mouse IgG1,κ。它的同型对照是纯化的Mouse IgG1,κ，货号是555746。它的二抗PE标记的抗小鼠IgG1,货号是550083。那么染色方式如下： 样本管：纯化的CDw25＋PE标记的抗Mouse IgG1＋