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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Eukaryotic translation initiation factor 1A, X-chromosomal (EIF1AX)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Eukaryotic translation initiation factor 1A, X-chromosomal (EIF1AX)品名简称:
Recombinant Human EIF1AX protein货号:
CSB-EP007506HU规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
EIF1AXUniprot No.:
P47813别名:
eIF-1A X isoform;Eukaryotic translation initiation factor 4C;eIF-4C种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
23.4 kDa表达区域:
1-144aa氨基酸序列:
MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCHIRGKLRKKVWINTSDIILVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDEDIDDI蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.功能2:
/文献:
/研究领域:
Epigenetics and Nuclear Signaling请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
Lee, S., Liu, B., Huang, S.X., Shen, B., and Qian, S.B. 2012. Global mapping of translation initiation sites in mammalian cells at single‐nucleotide resolution. Proc. Natl. Acad. Sci. U.S.A. 109:E2424‐E2432.
真核细胞蛋白质合成起始复合物的形成中需要更多的起始因子参与,因此起始过程也更复杂。 (1)需要特异的起始tRNA即,-tRNAfmet,并且不需要N端甲酰化。已发现的真核起始因子有近10种(eukaryote Initiation factor,eIF) (2)起始复合物形成在mRNA5’端AUG上游的帽子结构,(除某些病毒mRNA外) (3)ATP水解为ADP供给mRNA结合所需要的能量。真核细胞起始复合物的形成过程是:翻译起始也是由eIF-3结合在40S小亚基
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
protein), beta 1, 88kDa (CTNNB1), mRNA 1165 NM_003753 Homo sapiens eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa (EIF3S7), mRNA 1363 NM_004541 Homo sapiens NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa (NDUFA1
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