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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human High affinity immunoglobulin epsilon receptor subunit gamma (FCER1G)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human High affinity immunoglobulin epsilon receptor subunit gamma (FCER1G)品名简称:
Recombinant Human FCER1G protein货号:
CSB-EP008533HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
FCER1GUniprot No.:
P30273别名:
Fc receptor gamma-chain ;FcRgammaFc-epsilon RI-gammaIgE Fc receptor subunit gamma ;FceRI gamma种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
34.8 kDa表达区域:
19-86aa氨基酸序列:
LGEPQLCYILDAILFLYGIVLTLLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPPQ蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Associates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. Also involved in collagen-mediated platelet activation and in neutrophil activation mediated by integrin.功能2:
Associates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. Also involved in collagen-mediated platelet activation and in neutrophil activation mediated by integrin.文献:
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014)研究领域:
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文献和实验Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling
Fc receptor signaling plays a fundamental role in immune responses. A plethora of Fc �receptors (e.g., Fc gamma, Fc-alpha, and Fc-epsilon) are expressed on different immune cells, including natural killer cells, macrophages, mast cells
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
Marshall, J.G., Booth, J.W., Stambolic, V., Mak, T., Balla, T., Schreiber, A.D., Meyer, T., and Grinstein, S. 2001. Restricted accumulation of phosphatidylinositol 3‐kinase products in a plasmalemmal subdomain during Fc gamma receptor‐mediated
FcR阻断剂,让流式实验中的干扰无处遁形 说到Fc阻断,就不得不提到抗体。抗体的结构分为Fab和Fc段,Fab段是与目标分子特异性结合的区域,Fc段是Ig与效应分子或者细胞相互作用的部位[1](图1)。Fc受体(Fragment Crystallizable Receptor,FcR)是免疫调节受体中非常重要的受体,主要在先天免疫细胞上表达,能被抗体的Fc段识别并结合,从而引发免疫应答。 图1:抗体结构 为什么要做Fc阻断? 在细胞学实验中,正确区分阳性和阴性细胞十分重要。获得良好
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