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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见说明书
- 保质期:
见说明书
- 英文名:
Recombinant Human Coilin(COIL)
- 库存:
99
- 供应商:
武汉华美生物/CUSABIO
- 规格:
20ug/100ug/1mg
COIL蛋白|Recombinant Human Coilin(COIL)|大肠杆菌/毕赤酵母/哺乳动物细胞/杆状病毒-昆虫细胞/无细胞 五大表达系统
Uniprot ID:P38432
别名:CLN 80; CLN80; COIL; COIL_人; Coilin; Coilin p80; p80; P80 coilin
种属:Homo sapiens (Human)
氨基酸序列:参见官网
微信ID: Cusabio01 电话: 027-87939808
详情请登录:www.cusabio.cn
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文献和实验Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome
Transcriptional reporter systems have been extensively used to measure gene expression. Facile measurement of a reporter signal enables the study of gene expression in cases where protein product quantification is problematic. Reporters
与蛋白充分结合.其实加热不是问题的关键,比如上述的Reynolds等人制备SDS-protein样品的方法是用先用盐酸胍处理,以得到蛋白多肽的无规卷曲构象(random coil conformation)然后用含SDS与巯基乙醇的缓冲液进行透析,文中还指出,若是省去盐酸胍这一处理步骤,直接将蛋白用含SDS与巯基乙醇的缓冲液进行透析能得到一样的结合率(约1.4g/1g),同样在Reynolds的另一篇文献中(THE JOURNAL OF BIOLOGICAL CHEMISTRY,1970,245
Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells
not found in native protein. Fig. 1. Structure and functions of laminin-1 and it’s proteolytic derivatives. Laminins are heterotrimeric glycoproteins composed of α, β, and γ chains joined in a coiled-coil to form a cruciform-shaped molecule
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