Rabbit anti-Human Alkaline pho
sphatase, tissue-nonspecific isozyme protein polyclonal Antibody- 询价
- CUSABIO
- CSB-DA045GRN
- CN
- 2025年12月30日
- ELISA, Latex enhance immune than muddy, and other possible
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Rabbit anti-Human Alkaline phosphatase, tissue-nonspecific isozyme protein polyclonal Antibody
- 抗原：
IgG
- 应用范围：
ELISA, Latex enhance immune than muddy, and other possible
- 宿主：
Rabbit
- 级别：
诊断
- 适应物种：
human
- 供应商：
武汉华美
- 抗原来源：
human
- 保质期：
12个月
- 是否单克隆：
0
- 保存条件：
-20°C or -80°C
- 规格：
10mg
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文献和实验Human Alkaline Phosphatase staining of transgenic embryo/tissue
, penetration of stain may be improved by fixing in PBS + 0.2% glutaraldehyde + 0.02% NP-40 + 0.01% NaDC (at 4oC).Rinse in PBS. Heat inactivate endogenous alkaline phosphatase activity by incubation in PBS at 70-75oC for 30 minutes. Rinse in PBS at RT
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
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