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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
/
- 抗体英文名:
Rabbit anti-Sheep IgG Antibody;FITC conjugated
- 靶点:
/
- 浓度:
/
- 应用范围:
/
- 宿主:
Rabbit
- 适应物种:
Sheep
- 保质期:
6个月
- 抗原来源:
/
- 目录编号:
/
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 标记物:
FITC conjugated
- 克隆性:
Polyclonal
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
IgG purified from sheep serum
- 规格:
500μg
保存缓冲液
0.01M PBS, PH 7.4功能
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验对照。 二、注意事项 1、荧光染色后一般在 1h 内完成观察,或于 4℃ 保存 4h ,时间过长,可能会使荧光提前衰退。 2、每次试验均需设置以下三种对照: (1) 阳性对照:阳性血清+荧光标记物; (2)阴性对照:阴性血清+荧光标记物; (3)荧光标记物对照:PBS +荧光标记物。 三、免疫荧光双标的经验之谈 1、选取一抗时,要求来源于两种不同的动物,我用的是来源于家兔和大鼠的抗体,二抗则是不同荧光信号标记的,我用的是 donkey anti-rabbit-FITC(绿)和donkey anti
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
的价格比常规二抗 只高20%左右,但是Dylight的效价通常是常规荧光二抗的2-4倍左右,因此就最终的性价比,Dylight更胜一筹。举例来说,EarthOx的 FITC标记的山羊抗小鼠IgG二抗(货号为E031210),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的 Dylight 488标记的山羊抗小鼠IgG二抗(货号为E032210),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
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