手机验证
3-4周
博士德生物
酶联免疫竞争法
cell lysates,Plasma,Sample matrices,Urine
Mouse
96T
This ELISA kit is of competitive format. Competitive ELISA, also known as inhibition ELISA, is a surface/plate based assay, where the plate is coated with capture antibodies reactive to the molecule of interest. The sample (containing native molecule of interest) and enzyme conjugated recombinant protein (the competing molecule) are added to the coated wells. Since the amount of enzyme conjugated molecule in each well is constant, the level of native molecule in the sample will determine the binding ratio of enzyme conjugated molecule vs. native molecule. After an incubation period, any unbound antibody is washed off. Enzyme substrate (for example, TMB for HRP) is added to each well and will be transformed into a blue precipitate, the amount of which is linearly proportional to the amount of enzyme in the well. The precipitate is then turned into yellow by adding the acid stop solution and the concentration of yellow precipitate is read at 450nm for light absorbance (O.D. value). The O.D. is then used to calculate the amount of molecule of interest in each well, by comparing each sample well against the standard curve. The standard curve is generated using the same principle but instead of adding samples, a series of recombinant molecules with known concentrations are added to 6-8 wells.
Product Name | DNA Damage (8-OHdG) ELISA kit |
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SKU/Catalog Number | EK7008 |
Description | Competitive High Sensitivity ELISA kit for Quantitative Detection of 8-hydroxy-2-deoxy Guanosine. 96wells/kit |
Cite This Product | DNA Damage (8-OHdG) ELISA kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7008) |
Validated Species | Mouse |
Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. |
Cross Reactivity | 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%. |
Pack Size | 96wells/kit, with removable strips. |
Sensitivity | 0.59 ng/mL *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
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Assay Range | 0.94 - 60 ng/mL *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
Sample Type | Cell lysates, Plasma, Sample matrices, Urine *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
Storage | Store the kit at 4°C and -20°C. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Description | Quantity |
8-hydroxy-2-deoxy Guanosine : BSA Coated Plate | 12x8x1 Microwells |
8-hydroxy-2-deoxy Guanosine Standard | 1 vial/ 100uL |
8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody | 1 vial/75uL |
Sample and Standard Diluent | 1 vial/50mL |
8-hydroxy-2-deoxy Guanosine Antibody Diluent | 1 vial/13mL |
Wash Buffer Concentrate | 1 vial/50mL |
TMB Substrate | 1 vial/13mL |
Stop Solution | 1 vial/13mL |
Plate Cover | 2 covers |
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. ELISA plate reader capable of measuring at 450nm
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS.
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