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cell culture supernates, serum and plasma (heparin, EDTA).
Human
96T
货号 | EK1534 |
---|---|
产品名称 | Human Artemin PicoKine ELISA Kit |
产品描述 | For Quantitative Detection of human Artemin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,A108-G220 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | ARTN |
基因全名 | artemin |
Uniprot ID | Q5T4W7 |
指标别名 | Artemin; Enovin; Neublastin; ARTN; EVN |
背景资料 | Artemin, also known as enovin or neublastin, is a protein that in humans is encoded by the ARTN gene. This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1534-CAP | 96-well plate precoated with anti- human Artemin antibody | 1 |
EK1534-ST | Lyophilized recombinant human Artemin standard | 10ng/tube×2 |
EK1534-DA | Biotinylated anti- human Artemin antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 158 | 646 | 1984 | 149 | 615 | 1944 |
Standard deviation | 6.95 | 27.13 | 79.36 | 8.64 | 25.83 | 110.8 |
CV(%) | 4.4% | 4.2% | 4% | 5.8% | 4.2% | 5.7% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.008 | 0.084 | 0.146 | 0.263 | 0.501 | 0.888 | 1.540 | 2.016 |
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