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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and saliva.
96T
货号 | EK0532 |
---|---|
产品名称 | Human TRAIL ELISA Kit |
产品描述 | For quantitative detection of human TRAIL in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and saliva. |
免疫原 | NSO,T95-G281 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | TNFSF10 |
基因全名 | tumor necrosis factor (ligand) superfamily, member 10 |
Uniprot ID | P50591 |
指标别名 | Apo 2 ligand|APO2L|CD253|Protein TRAIL|TL2|TNF related apoptosis inducing ligand|Tumor necrosis factor ligand superfamily member 10 |
背景资料 | In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0532-CAP | 96-well plate precoated with anti- human TRAIL antibody | 1 |
EK0532-ST | Lyophilized recombinant human TRAIL standard | 10ng/tubex2 |
EK0532-DA | Biotinylated anti- human TRAIL antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 38 | 184 | 416 | 34 | 175 | 377 |
Standard deviation | 2.66 | 9.56 | 18.72 | 2.75 | 9.62 | 18.85 |
CV(%) | 7% | 5.2% | 4.5% | 8.1% | 5.5% | 5% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.066 | 0.123 | 0.166 | 0.303 | 0.509 | 0.824 | 1.549 | 2.458 |
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