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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1120 |
---|---|
产品名称 | Human CCL15 ELISA Kit |
产品描述 | For quantitative detection of human CCL15 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, Q22-I113 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | CCL15 |
基因全名 | chemokine (C-C motif) ligand 15 |
Uniprot ID | Q16663 |
指标别名 | CC chemokine 3|CCL15|CCL15(29-92)|CCL15_HUMAN|Chemokine CC-2|Chemokine CC2|HCC-2|HCC2|HMRP 2B|HMRP2B|Leukotactin 1|Leukotactin-1|LKN-1|LKN1|Macrophage inflammatory protein 5|MIP-1 delta|MIP-5|MIP1 delta|MIP1d|MIP5|Mrp-2b|Mrp2b|NCC-3|NCC3|SCYA15|SCYL3|Small inducible cytokine A15|Small inducible cytokine subfamily A (Cys-Cys), member 15|Small-inducible cytokine A15|SY15 |
背景资料 | Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1120-CAP | 96-well plate precoated with anti- human CCL15 antibody | 1 |
EK1120-ST | Lyophilized recombinant human CCL15 standard | 10ng/tube×2 |
EK1120-DA | Biotinylated anti- human CCL15 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 185 | 439 | 31 | 188 | 417 |
Standard deviation | 2.31 | 9.8 | 31.6 | 2.75 | 13.34 | 37.11 |
CV(%) | 7% | 5.3% | 7.2% | 8.9% | 7.1% | 8.9% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.042 | 0.092 | 0.121 | 0.194 | 0.319 | 0.570 | 1.023 | 2.010 |
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