Plasma/Serum RNA Purification

产品名称：血浆/血清RNA纯化大提试剂盒（纯化）-Plasma/Serum RNA Purification Maxi Kit

产品货号：56200

产品规格：10次

背景资料：Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 - 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.

保存建议：血浆/血清RNA纯化大提试剂盒（纯化）-Plasma/Serum RNA Purification Maxi Kit溶液密封室温(15-25°C)保存，未开封至少保存2年。具体保存情况收到产品后请按说明存放。

产品描述：This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

点击：血浆/血清RNA纯化大提试剂盒（纯化）-Plasma/Serum RNA Purification Maxi Kit查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询Norgen Biotek中国区域独家代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看每日生物评论网站。

Norgen Biotek成立于1998年，公司总部位于加拿大，是一家著名全球化生物技术公司，基于专利的碳化硅膜纯化技术和无苯酚专利配方，生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖，是一家致力于样品制备并获得ISO 9001（产品质量）、ISO 13485（产品可用于商业化的医疗设备，包括用于体外诊断领域）和ISO 15189（可用于临床检测机分子诊断领域的研发能力）认证的生物科技公司。目前该公司的产品多达700余种，提供一流的样品制备试剂盒，应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA，RNA和蛋白质的纯化富集，从活检样本（包括尿液，血浆，血清和血液）中纯化RNA和DNA，全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。

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关键词：细胞分析试剂盒，血浆/血清RNA纯化大提试剂盒（纯化），血浆/血清RNA纯化大提试剂盒（纯化）