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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Direct Black 38;Chlorazol Black E;Disodium 4-amino-3-[[4'-[(2,4-diaminophenyl)azo][1,1'-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)naphthalene-2,7-disulphonate
- CAS号:
1937-37-7
- 保质期:
2年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
RT
- 规格:
瓶
| [产品编号] | LM1297S |
| [CAS号] | 1937-37-7 |
| [规格] | BR,70% |
| [包装] | 5克/25克 |
| [到货期] | 1-2天 |
英文名称:Direct Black 38;Chlorazol Black E;Disodium 4-amino-3-[[4'-[(2,4-diaminophenyl)azo][1,1'-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)naphthalene-2,7-disulphonate
其他名称:直接黑EX;直接青光元;直接元;直接元青;氯唑黑;卡拉唑黑E;酸性绿光直接黑;直接黑38
CAS号:1937-37-7
C34H25N9Na2O7S2=781.73
级别:BR
含量:≥70.0%
性状(以下信息仅供参考):深褐色或黑色粉末,溶于水
用途:本品仅供科研,不得用于其它用途
保存:RT
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文献和实验spread, cover with parafilm (acting as a coverslip) and incubate in a humid chamber at 37o C for 12-16 hours (4) Post-hybridisation treatment / detection (a) wash: 40% formamide (standard grade) in 2 x SSC at 45o C for 10
% formamide (standard grade) in 2 x SSC at 45o C for 10 minutes 2 x SSC at 45o C for 10 minutes 2 x SSC at room temperature for 10 minutes (b) immersion and blocking: BN buffer (0.1M NaHCO3 / 0.1% NP-40) or BI buffer (0.1M NaHCO
conjugate (Boehringer Mannheim) (800 ng in blocking buffer) and incubate at 37oC for 30 minutes in the dark. wash with excess BN buffer on a shaker at room temperature to remove unbound conjugate mount in 20 µl of anti-fade solution containing
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