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500/860 TOC Annual Pharma Stds. Kit, monthly
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
transfer supernatant to a new 2 ml tube and add100 ul HTR Reagent. Mix throughly by vortexing for 10 seconds. 7. Incubate at room temperature for 2 minutes. Centrifuge at full speed (13,000 x g) for 2 minutes. 8. Transfer 500 ul
Fastfilter Plasmid Midi Kit Spin Protocol
2. Pellet up to 30-50 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 x g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel to blot
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