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- 2025年03月19日
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文献和实验Using ChemBank to Probe Chemical Biology
Pathways and Processes Basic Protocol 4: Dissect Small‐Molecule Structure Using Assay Profiles Basic Protocol 5: Identify Structurally Related Small Molecules with Known Biological Functions
The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
Design of the Invader Genotyping Assay
_MAX_GC=70 PRIMER_MAX_POLY=3 PRIMER_NUM_RETURN=1000 Invader Assay Design1. To design an Invader assay for SNP genotyping, the sequence of 40-50 bases on each side of the polymorphic site on the target must be known. Although either the sense
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