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文献和实验[资源] 申请上传 Current Protocols In Protein Science (60M) 见公共邮箱
from Escherichia coli Unit 6.4 Overview of Protein Folding Unit 6.5 Folding and Purification of Insoluble (Inclusion Body) Proteins from Escherichia coli Unit 6.6 Expression and Purification of GST Fusion Proteins Unit 6.7 Expression and Purification
High‐Throughput Screening for Protein‐Protein Interactions Using Yeast Two‐Hybrid Arrays
., ) PJ69‐4α (MATα trp1‐901 leu2‐3,112 ura3‐52 his3‐200 gal4Δ gal80Δ LYS2::GAL1‐HIS3 GAL2‐ADE2 met2::GAL7‐lacZ; Uetz et al., ) 0.1 M lithium acetate
Protein Secondary Structure Prediction
Baldwin, J.M. 1993. The probable arrangement of helixes in G protein–coupled receptors. EMBO J. 12:1693‐1703. Blundell, T.L. and Johnson, L.N. (eds.) 1976
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