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文献和实验Expand High Fidelity PCR--高保真PCR
Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products
SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
. Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. Platinum® Taq DNA Polymerase High Fidelity is an enzyme mixture composed of recombinant Taq DNA polymerase
The Polymerase Chain Reaction (PCR)
Topics • What is the Polymerase Chain Reaction? • History and (pre-history) of PCR • How PCR works • Optimising PCR • Fidelity, errors and cloning • PCR primer design • Applications of PCR What is the Polymerase Chain Reaction? • It’s
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