DNA Size Standard Lambda Ladde

Construction of Lambda Libraries from Large PFGE Fragments

Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fragments of DNA up to thousands of kilobases in size. This aspect of the technique has been exploited for constructing long-range restriction maps of chromosomes

Separation and Size Determination of DNA over a 10,200 kbp Range

7 ). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range

Standard PCR reaction

below Tm) Step 4: Extend DNA for 2 minutes at 72o C Step 5: Repeat steps 2-4 for 25-30 cycles Step 6: Final Extension for 10 minutes at 72o C   Run 2 μL on a gel to check size and concentration of PCR product.