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文献和实验Separation and Size Determination of DNA over a 10,200 kbp Range
7 ). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range
Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis
Standard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40–50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE
in the bottom of the well. Materials DNA samples DNA size standard Loading dye solution Agarose TBE buffer Sybr Green I solution diluted 10,000-fold Dark Reader light box Plastic wrap
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