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文献和实验freeshotgun sequencing library from miniprep BAC DNA
at 37℃. 7.Purify insert DNA with PFGE Add 60 μl of 6X DNA loading buffer into digestion mixture, load on 1% agarose - 0.5X TBE gel, run at 6V/cm, initial pulse 5 seconds and final pulse 15 seconds for 18 hrs with BioRAD CHEF-DR (r) II
BAC DNA Purification For Injection
This is a two step procedure, utilizing a Qiagen Large-Construct Kit for the initial purification, followed by either a chromatographic separation OR gel purification. Qiagen Prep of BAC DNA Modified from Biotechniques 27:72-74 (1999
free shotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA 1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramphenicol with BAC clone and grow oveernight. Miniprep with AutoGen 740 according to the protocol described in our Web page. DNA pellet were resuspended in 265 μl TE
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