ZE5 Additive, 4x300ml

PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)

of amplification, as the mean length of target DNA is decreased: for templates of 300bp or less, denaturation temperature may be reduced to as low as 88℃ for 50% （G+C） templates （Yap and McGee, 1991）， which means one may do as many as 40 cycles

Assay of superoxide dismutase activity

for the assay of SOD activity, 3 g of rice seedlings, tobacco and papaya young leaves were harvested and frozen with liquid nitrogen, ground into a fine powder, and then mixed with 3 ml of extraction buffer (0.15 M Tris, pH 7.5). The samples were centrifuged

流式检测凋亡的讨论

： 100 Tests 有 效 期： 见外包装盒 试剂盒组份 1、结合缓冲液 4x (Binding Buffer 4x)：体积：20ml（4倍浓缩液）； 稀释后溶液中各组分浓度：10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2 2、碘化丙锭溶液 （Propidium Iodide）体积： 1.0ml；浓度：20ug/ml 3、重组人Annexin V/FITC, (rh Annexin V/FITC) 来 源：大肠杆菌（E.coli） 分