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12个月
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采用精密的电子烟(或卷烟)烟雾发生器和机械控制单元,满足国家电子烟标准与国际标准(例如:GB 41700-2022《电子烟》、法国XP D90-300-3)的电子烟实验抽吸方法,通过产生烟气,使动物持续吸入,用于电子烟(或卷烟)烟气毒理实验,可以建立小鼠、大鼠、豚鼠的COPD等相关疾病模型。
性能特点:
烟气评价多样化,采用电子烟抽吸、或选配电子烟烟液加热蒸发或选配卷烟抽吸
自动化吸烟,提高实验的一致性和可重复性
烟雾管道易于拆卸,便于清洗或更换
产生的气溶胶粒径为肺部可沉积范围
暴露腔采用透明材质,方便观察
精密流量计可以便于调节烟气浓度
配有烟气浓度检测模块,全面监测烟气暴露
具有废气处理模块,达到实验室安全排放要求
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文献和实验Anti-fatigue
effect
and
acute
toxicity
test
of
D-borneol
refreshing
atomization
solution
on
mice
Tian
Xinhui1,
Chen
Hongbo2,
Cheng
Fang2,
Han
Yuhang2,
Yang
Xinrui2,
Su
Dandan2,
Li
Siqi1,
Huang
Xiaoling1,
Liu
Chunyan4,
Pang
Yuxin1,3∗
(1.
School
of
Traditional
Medicine
Materials
Resource,
Guangdong
Pharmaceutical
University,
Yunfu
527322,
Guangdong
Province,
China;
2.
School
of
Pharmaceutical
Sciences,
Shenzhen
Campus
of
Sun
Yat-sen
University,
Shenzhen
518107,
Guangdong
Province,
China;
3.
School
of
Pharmacy,
Guizhou
University
of
Traditional
Chinese
Medicine,
Guiyang
550025,
China;
4.
School
of
Agronomy,
Henan
Agricultural
University,
Zhengzhou,
China
450046,
China)
【Abstract】 Objective
To
investigate
the
anti-fatigue
effects
and
acute
toxicity
of
different
doses
of
D-borneol
refreshing
atomization
solution
on
mice,
and
to
provide
reference
for
the
further
development
and
application
of
D-borneol
refreshing
atomization
solution
in
clinic.
Methods Sixty
specifc
pathogen
free
(SPF)
male
KM
mice
were
divided
into
one
blank
group
and
four
dose
groups,
with
12
mice
in
each
group,
for
acute
toxicity
experiments
and
weight-bearing
swimming
experiments.
In
the
acute
toxicity
test,
the
toxic
reaction
of
mice
was
observed.
After
dissection,
histopathological
examination
was
performed.
For
weight-bearing
swimming
experiment,
serum,
liver
and
hind
leg
muscle
tissues
of
mice
were
collected.
Blood
glucose
(Glu),
lactic
acid
(LA),
blood
urea
nitrogen
(BUN),
and
lactate
dehydrogenase
(LDH)
activity
levels
were
measured
in
serum.
Liver
glycogen
(LG)
content
was
assessed
in
liver
tissue;
muscle
glycogen
(MG)
and
other
anti-fatigue
biochemical
indicators
were
evaluated
in
muscle
tissue.
Malondialdehyde
(MDA)
content
and
the
activities
of
superoxide
dismutase
(SOD)
and
glutathione
peroxidase
(GSH-Px)
were
determined
in
muscle
tissue.
Results During
the
administration
of
D-borneol
refreshing
atomization
solution,
no
abnormalities
were
observed
in
the
mental
state,
fur
color,
or
body
weight
of
mice
across
all
groups
during
the
acute
toxicity
test.
Pathological
examinations
did
not
reveal
any
abnormalities
either.
The
weight-
bearing
swimming
test
results
demonstrated
that
medium
and
high
doses
of
D-borneol
refreshing
atomization
solution
could
effectively
prolong
the
time
of
weight-bearing
exhaustive
swimming
in
mice
(P<0. 001),
significantly
increase
the
content
of
MG
(P<0. 01),
and
测定。参考GB/T14551-93,选用电子俘获检测器,配以毛细管进样系统和专用大口径毛细管柱,可完成对茶叶中有机氯农药残留的检测。 (六)烟草及烟草制品检测分析: 选用TCD、FID,配以专用色谱柱,可完成对烟气总粒相物中水份及尼古丁含量的检测,其方法是国际上普遍采用的一种快速、准确、先进的测试方法。对烟草、烟草制品中有机氯、有机磷、拟除虫菊酯等农药残留的测定,可采用ECD、FPD、NPD检测器配以不同的毛细管柱来完成。可参考国标GB/T13595-2004和GB/T
-2003)和动物性食品(GB/T5009.162-2003)。海产品中多氯联苯的气相色谱法(GB/T5009.190-2003)。食品中氯丙醇的检验,可采用三氯乙酐衍生化结合电子俘获检测器(ECD)进行测定。参考GB/T14551-93,选用电子俘获检测器,配以毛细管进样系统和专用大口径毛细管柱,可完成对茶叶中有机氯农药残留的检测。 (十四)烟草及烟草制品检测分析: 选用TCD、FID,配以专用色谱柱,可完成对烟气总粒相物中水份及尼古丁含量的检测,其方法是国际上普遍采用的一种快速
aerosols 悬浮于气体介质中的粒径一般为0.001微米~100微米的固态或液态微小粒子形成的相对稳定的分散体系。 2.6 生物安全 biosafety 避免危险生物因子造成实验室人员暴露,向实验室外扩散并导致危害的综合措施。 2.7 高效空气过滤器 high efficiency particulate air filter(HEPA) 通常以滤除大于等于0.3微米微粒为目的,滤除效率符合相关要求的过滤器。 2.8 安全罩 safety
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