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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
Detection and Immobilization of Proteins Containing the 6xHis Tag
Many systems have been developed for the heterologous expression of recombinant proteins. They are often based on the fusion of the protein of interest with a naturally occurring protein (glutathione S -transferase [1 ], maltose binding protein
spa Typing for Epidemiological Surveillance of Staphylococcus aureus
The spa typing method is based on sequencing of the polymorphic X region of the protein A gene (spa ), present in all strains of Staphylococcus aureus. The X region is constituted of a variable number of 24-bp repeats flanked
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