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文献和实验and describe two advanced protocols that make use of the membrane binding dye FM 1-43, enabling to probe deep into the mechanisms of synaptic vesicle function and dynamics at the Drosophila third instar larval neuromuscular junction.
Isolation of CP43 and CP47 Photosystem II Proximal Antenna Complexes From Plants
A single-column method to purify the CP43 and CP47 pigment-protein complexes of photosystem (PS)II from higher plants is presented. To validate the isolation procedure, three different species were used (Spinacea oleracea, Beta vulgaris
Quantitative Profiling of Protein-DNA Binding on Microarrays
on particular motifs. Thus, there is an urgent need for systematic profiling of vertebrate transcription factor binding to DNA. We have developed a high-throughput platform for the quantitative analysis of protein-DNA interactions based on microarray technology.
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