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- 详细信息
- 技术资料
- 免疫原:
Culture supernatant of E. coli 0111 ( EHEC strain)
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Campylobacter jejuni, E. coli, Staphylococcus aureus, Salmonella Enteritidis
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
E. coli
- 目录编号:
GTX00863
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA, Lateral Flow
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
It reacts with subunit A of VT1, VT2 and Stx.
- 抗体英文名:
Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]
- 抗体名:
Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A 抗体 [vero-01]
- 规格:
100 μg
Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.
WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2.
Lane 1 : Culture medium of E. coli O157:H7
Lane 2 : Purified VT1
Lane 3 : Purified VT2
Lane 4 : VT non-producing E. coli
ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution
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